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Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform |
Synonyms: | PI3-kinase subunit alpha, PI3K-alpha, PtdIns-3-kinase subunit alpha, p110alpha, PIK3CA, PtdIns-3-kinase subunit p110-alpha |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human PIK3CA |
Uniprot ID: | P42336 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cell membrane |
Predicted MW. / Observed MW. | 124 kDa / 110 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, hippocampus |
ICC/IF | Human, Mouse | 1: 50-1: 100 | NIH-3T3 |
Background
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).
Images
| WB analysis of PIK3CA(GB112544). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of PIK3CA(GB112544)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
