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| Reactivity: | H & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PKC rabbit polyclonal |
Gene Name: | Protein kinase C epsilon type |
Synonyms: | nPKC-epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse PKC |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton, Nucleus, Cell membrane |
| Predicted MW. / Observed MW. | 84 kDa / 100 kDa |
Uniprot ID: |
Product Usage Information
WB | Human | 1: 500-1: 1000 | HeLa, U-251 |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 800 | kidney, brain |
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
Images
| Western blot analysis of PRKCE (GB113782) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human kidney using PRKCE (GB113782) at dilution of 1: 700 |
| Immunohistochemistry analysis of paraffin embedded mouse brain using PRKCE (GB113782) at dilution of 1: 700 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
