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Anti-PPAR alpha Rabbit pAb (100 μl)
| Reactivity: | |
| Applications: | Elisa |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Peroxisome proliferator-activated receptor alpha |
Synonyms: | PPARA, NR1C1, PPAR, PPARalpha, hPPAR, peroxisome proliferator activated receptor alpha, PPARα |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse PPAR alpha |
Uniprot ID: | P23204 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Product Usage Information
IHC/IF | Mouse, Rat | 1: 200-1: 800 | colon, kidney |
Background
Peroxisome proliferator-activated receptor alpha (PPAR-alpha), also known as NR1C1, is a nuclear receptor protein that in humans is encoded by the PPARA gene.
PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver. PPAR-alpha is activated under conditions of energy deprivation and is necessary for the process of ketogenesis, a key adaptive response to prolonged fasting.[7] Activation of PPAR-alpha promotes uptake, utilization, and catabolism of fatty acids by upregulation of genes involved in fatty acid transport, fatty acid binding and activation, and peroxisomal and mitochondrial fatty acid β-oxidation.
Images
| IHC analysis of PPAR alpha (GB11163). Sample: Mouse colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of PPAR alpha (GB11163). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of PPAR alpha (GB11163). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of PPAR alpha (GB11163). Sample: Rat liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
