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Anti-PRP19 Rabbit pAb (100 μl)
| Reactivity: | H & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Pre-mRNA-processing factor 19 |
Synonyms: | Nuclear matrix protein 200, PRP19/PSO4 homolog, RING-type E3 ubiquitin transferase PRP19, Senescence evasion factor, Prp19, Snev, Prpf19 |
Immunogen: | Recombinant protein corresponding to Mouse PRP19 |
Uniprot ID: | Q99KP6 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 55 kDa / 55 kDa |
Product Usage Information
WB | Human | 1: 1000-1: 2000 | HeLa, A-431, MCF7, Jurkat, NIH/3T3 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | brain, kidney |
Background
Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response.
Images
| Western blot analysis of PRP19 (GB111808) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat lung using PRP19 (GB111808) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded rat kidney using PRP19 (GB111808) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded mouse lung using PRP19 (GB111808) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded mouse brain using PRP19 (GB111808) at dilution of 1: 500 |
| Immunofluorescent analysis of paraffin embedded rat brain using PRP19 (GB111808) at dilution of 1: 500 |
| Immunofluorescent analysis of paraffin embedded mouse brain using PRP19 (GB111808) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
