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Anti-PSTPIP1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Proline-serine-threonine phosphatase-interacting protein 1 |
Synonyms: | CD2 binding protein 1, CD2BP1, CD2BP1L, CD2BP1S, H PIP, PAPAS, PSTPIP, PSTPIP1, PEST phosphatase-interacting protein 1, HPIP |
Immunogen: | Recombinant protein corresponding to Mouse PSTPIP1 |
Uniprot ID: | P97814 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 48 kDa / 48 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 600-1: 1200 | thymus, brain, spleen |
ICC/IF | Human, Mouse | 1: 50-1: 200 | HeLa, NIH-3T3 |
Background
PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), also known as CD2BP1, is a cytoskeleton-associated adaptor protein that links PEST-type phosphatases to their substrates . It is involved in regulation of the actin cytoskeleton. Preferentially expressed in hematopoetic tissues, PSTPIP1 binds to the cytoplasmic tail of CD2, negatively affecting CD2-triggered T cell activation . It has also been shown to interact with ABL1, PTPN18, WAS, CD2AP, PTPN12 and MEFV/pyrin. Mutations in the gene of PSTPIP1 cause PAPA syndrome (Pyogenic sterile Arthritis, Pyoderma gangrenosum, and Acne), an autoinflammatory disease.
Images
| WB analysis o f PSTPIP1(GB113289). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1200, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of PSTPIP1(GB113289)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of PSTPIP1(GB113289)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
