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Anti-PUF60/FIR Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PUF60/FIR rabbit polyclonal |
Gene Name: | Poly(U)-binding-splicing factor PUF60 |
Synonyms: | FBP interacting repressor, FIR, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse PUF60/FIR |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 60 kDa |
Observed MW. | 65kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 300-1: 800 | thymus |
IHC/IF | Mouse, Rat | 1: 300-1: 600 | brain, pancreas |
Background
Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.
Images
| Western blot analysis of PUF60 (GB113774) at dilution of 1: 800 |
| Western blot analysis of PUF60 (GB113774) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin-embedded mouse brain using PUF60 (GB113774) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded rat brain using PUF60 (GB113774) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded mouse pancreas using PUF60 (GB113774) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded rat pancreas using PUF60 (GB113774) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
