Reactivity: | M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Peripherin |
Synonyms: | Prph, Prph1, Neurofilament 4, NEF4, Perf |
Immunogen: | Recombinant protein corresponding to Mouse Peripherin |
Uniprot ID: | P15331 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoskeleton |
Predicted MW. / Observed MW. | 54 kDa / 54 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | spinal cord, colon |
IHC/IF | Mouse, Rat | 1: 800-1: 3000 | colon |
Background
Peripherin is a 57kD type III intermediate filament that is a specific marker for peripheral neurons, including enteric ganglion cells. Peripherin is expressed in the developing peripheral nervous system and is highly enriched in neuronal derivatives of the neural crest. Peripherin offers an advantage over other neural markers, such as S100 and NSE in that it does not stain chrmaffin cell types.
Images
Western blot analysis of peripherin (GB111635) at dilution of 1: 1000 | |
| Immunohistochemistry analysis of paraffin embedded rat colon using peripherin (GB111635) at dilution of 1: 1600 |
Immunohistochemistry analysis of paraffin embedded mouse colon using peripherin (GB111635) at dilution of 1: 1600 | |
Immunofluorescent analysis of paraffin embedded rat colon using peripherin (GB111635) at dilution of 1: 1600 | |
Immunofluorescent analysis of paraffin embedded mouse colon using peripherin (GB111635) at dilution of 1: 1600 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |