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Anti-Phospho-FOXO1 (S256) Rabbit pAb (100 μl)
| Reactivity: | H,M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Phospho-FOXO1(S256) rabbit polyclonal antibody |
Gene Name: | Forkhead box protein O1 |
Synonyms: | FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A, P-FOXO1 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human FOXO1(S256) |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 70 kDa |
Observed MW. | 82 kDa |
Uniprot ID: | Q12778 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1: 300-1: 600 | 239, C2C12 |
Background
FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins.
Images
 | Western blot analysis of phospho-FOXO1(S256) (GB113974) at dilution of 1: 600 Lane1: 293 untreated, whole cell lysate Lane2: 293 was starved overnight and then treated with 20% FBS, whole cell lysate Lane3: C2C12 untreated, whole cell lysate Lane4: C2C12 was treated with 100nmol/L insulin for 15min, whole cell lysate |
 | Western blot analysis of phospho-FOXO1(S256) (GB113974) at dilution of 1: 600 Lane1: 293 untreated, whole cell lysate Lane2: 293 was starved overnight and then treated with 20% FBS, whole cell lysate Lane3: C2C12 untreated, whole cell lysate Lane4: C2C12 was treated with 100nmol/L insulin for 15min, whole cell lysate The figure above was treated with non-phospho-blocking pepide, the figure below was treated with phospho- blocking pepide |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
