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Recombinant Anti-Phospho-STAT3 (Y705) Rabbit mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Signal transducer and activator of transcription 3 |
Synonyms: | APRF, ADMIO, APRF, HIES, ADMIO1, Acute-phase response factor, STAT3 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human STAT3(Tyr705) |
Uniprot ID: | P40763 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 79/86 kDa / 79/86 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | C2CL2,HeLa |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | renal carcinoma, subcutaneous nude-mouse model of 4T1 cell |
Background
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors.Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene.May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes.
Images
| WB analysis of Phospho-STAT3(Tyr705)(GB150001). |
| WB analysis of Phospho-STAT3(Y705)(GB150001). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of Phospho-STAT3(Y705)(GB150001). Sample: Human renal carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Phospho-STAT3(Y705)(GB150001). Sample: Mouse subcutaneous nude-mouse model of 4T1 cell, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Phospho-STAT3(Y705)(GB150001). Sample: Rat Inflammatory model induced by lps(6h) lung, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
