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Anti-Phospho-YAP1 (S127) Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Phospho-YAP1(S127) rabbit polyclonal antibody |
Gene Name: | Transcriptional coactivator YAP1 |
Synonyms: | 65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog, P-YAP1 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to human YAP1(S127) |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 48-54 kDa |
Observed MW. | 78 kDa |
Uniprot ID: | P46937 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 300-1: 500 | SW480, HepG2, NIH/3T3, C6 |
Background
Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration.
Images
 | Western blot analysis of Phospho-YAP1(S127) (GB114060) at dilution of 1: 500 |
 | Western blot analysis of Phospho-YAP1(S127) (GB114060) at dilution of 1: 500 The figure above was treated with phospho-blocking pepide, the figure below was treated with non-phospho- blocking pepide |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
