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Anti-Prohibitin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Prohibitin rabbit polyclonal |
Gene Name: | Prohibitin |
Synonyms: | B-cell receptor-associated protein 32, BAP 32, Phb, PHB1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Prohibitin |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 30 kDa |
Observed MW. | 30 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 2000-1: 4000 | brain, liver, heart, kidney |
IHC | Human | 1: 900-1: 1800 | liver, kidney |
Background
Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis . This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription . Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis .
Images
| Western blot analysis of Prohibitin (GB113098) at dilution of 1: 2000 Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF-7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Mouse liver tissue lysate Lane 8: Rat brain tissue lysate Lane 9: Rat liver tissue lysate |
| Western blot analysis of Prohibitin (GB113098) at dilution of 1: 2000 Lane 1: MCF-7 cell lysate Lane 2: Jurkat cell lysate Lane 3: PC-12 cell lysate Lane 4: Mouse heart tissue lysate Lane 5: Mouse liver tissue lysate Lane 6: Mouse kidney tissue lysate Lane 7: Rat heart tissue lysate Lane 8: Rat liver tissue lysate Lane 9: Rat kidney tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded human liver using Prohibitin (GB113098) at dilution of 1: 1800 |
| Immunohistochemistry analysis of paraffin-embedded human kidney using Prohibitin (GB113098) at dilution of 1: 1800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
