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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Receptor-interacting serine/threonine-protein kinase 3 |
Synonyms: | Receptor interacting protein 3, RIP 3, RIP like protein kinase 3, RIP3, RIPK3 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human RIP3 |
Uniprot ID: | Q9Y572 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 57 kDa / 57 kDa |
Product Usage Information
WB | Human | 1: 300-1: 500 | THP-1, HT-29, Jurkat, SK-BR-3 |
Background
Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (By similarity).
Images
| Western blot analysis of RIP3 (GB115458) at dilution of 1: 300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
