Reactivity: | H,M,R & H,M,R |
Applications: | WB & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | RPL34 rabbit polyclonal antibody |
Gene Name: | 60S ribosomal protein L34 |
Synonyms: | Rpl34, L34, Ribosomal protein L34 |
Immunogen: | Recombinant protein corresponding to Mouse RPL34 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 13 kDa |
Observed MW. | 13 kDa |
Uniprot ID: | P49207, Q9D1R9, P11250 |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | thymus |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa |
Background
This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Images
Western blot analysis of RPL34 (GB112367) at dilution of 1: 500 | |
ICC analysis of RPL34(GB112367)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. | |
ICC analysis of RPL34(GB112367)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |