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Anti-RPS15 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 40S ribosomal protein S15 |
Synonyms: | RIG protein, Rps15, Rig, ribosomal protein S15 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RPS15 |
Uniprot ID: | P62843 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 17 kDa / 17 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | thymus, lung, brain |
ICC/IF | Mouse | 1: 50-1: 200 | HeLa, NIH-3T3 |
Background
RPS15, also named as RIG protein or Small ribosomal subunit protein uS19, is a 145 amino acid protein, which belongs to the universal ribosomal protein uS19 family. RPS15 is a ribosomal small subunit assembly and involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay.
Images
| WB analysis of RPS15(GB111967). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody:: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of RPS15(GB111967)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of RPS15(GB111967)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
