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Anti-RPS25 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 40S ribosomal protein S25 |
Synonyms: | RPS25, S25 |
Immunogen: | Recombinant protein corresponding to Mouse RPS25 |
Uniprot ID: | P62852 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 14 kDa / 16 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, lymph node, uterus |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1500 | breast cancer, colon cancer |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa |
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S25E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Images
| Western blot analysis of RPS25 (GB112141) at dilution of 1: 1000 |
| Immunofluorescence analysis of paraffin embedded human breast cancer using RPS25 (GB112141) at dilution of 1: 500 |
| Immunofluorescence analysis of paraffin embedded human colon cancer using RPS25 (GB112141) at dilution of 1: 500 |
| ICC analysis of RPS25(GB112141)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
