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Anti-RPS3A Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 40S ribosomal protein S3a |
Synonyms: | Fte-1, FTE1, MFTL, ribosomal protein S3A, RPS3A, Protein TU-11, Rps3a1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RPS3A |
Uniprot ID: | P97351 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 30 kDa / 35 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | liver, pancreas |
ICC/IF | Human, Mouse | 1: 50-1: 100 | HeLa,NIH-3T3 |
Background
RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors.
Images
| WB analysis of RPS3A(GB113862). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of RPS3A(GB113862)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of RPS3A(GB113862)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
