Reactivity: | M |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Mitochondrial RNA pseudouridine synthase Rpusd4 |
Synonyms: | FLJ14494 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RPUSD4 |
Uniprot ID: | Q9CWX4 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 42 kDa / 42 kDa |
Product Usage Information
WB | Mouse | 1: 1000-1: 2000 | liver, spleen, testis |
Background
Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome. Acts on position 39 in mitochondrial tRNA(Phe). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation.
Images
Western blot analysis of RPUSD4 (GB115261) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |