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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Raf1 rabbit polyclonal antibody |
Gene Name: | RAF proto-oncogene serine/threonine-protein kinase |
Synonyms: | RAF1, Raf-1 proto-oncogene, Serine/threonine kinase, CMD1NN, CRAF, Proto-oncogene c-RAF, NS5, Raf-1, C-Raf |
Immunogen: | Recombinant protein corresponding to Mouse c-Raf |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 73 kDa |
Observed MW. | 73 kDa |
Uniprot ID: | P04049, Q99N57, P11345 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1:500-1:1000 | lung, kidney, testis |
Background
RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF or simply c-Raf or even Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. The c-Raf protein is part of the ERK1/2 pathway as a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras subfamily of membrane associated GTPases. C-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases, from the TKL (Tyrosine-kinase-like) group of kinases. Although c-Raf is very clearly capable of mutating into an oncogene in experimental settings, and even in a few human tumors, its sister kinase B-Raf is the true major player in carcinogenesis in humans.
Images
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Western blot analysis of Raf1 (GB11543) at dilution of 1: 500
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Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
