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Anti-Rsk 2 / MAPKAP Kinase 1b Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Ribosomal protein S6 kinase alpha-3 |
Synonyms: | CLS, HU 3, ISPK 1, ISPK1, MAPKAP kinase 1b, MAPKAPK 1b, MAPKAPK1B, MRX19, p90 RSK 3, p90 RSK2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Rsk2 |
Uniprot ID: | P18654 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 50-1: 150 | testis, liver, lung |
Background
Serine/threonine-protein kinase that acts downstream of ERK signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation , phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway.
Images
| Immunohistochemistry analysis of paraffin embedded human colon using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded human esophageal using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded human Liver cancer using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded human lung cancer using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded mouse skeletal muscle using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded mouse testis using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded rat colon using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded rat pancreas using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded rat skeletal muscle using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded rat testis using Rsk 2 / MAPKAP Kinase 1b (GB115595) at dilution of 1:150 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
