Synonyms: | KETAAAKFERQHMDS tag, S peptide epitope tag, S peptide, S15, S-Tag, S-tag, S TAG | Immunogen: | KLH conjugated Synthetic peptide corresponding to S-tag:KETAAAKFERQHMDS | Isotype: | IgG1 | Purity: | Affinity purification |
Product Usage Information Applications | Species | Dilution | Positive Tissue | WB | Species independent | 1: 500-1: 1000 | S-tagged fusion protein 1, S-tagged fusion protein 2, 293FT, Transfected 293FT |
Background Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The S tag is corresponds to amino acid residues KETAAAKFERQHMDS of pancreatic ribonuclease A (RNase A). Many recombinant proteins have been engineered to express the S tag. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to.
Images 
| WB analysis of S Tag(GB150008).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. | 
| WB analysis of S Tag(GB150008).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
Storage | Storage
|
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. | | Storage Buffer
|
PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
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