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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SAE1 rabbit polyclonal antibody |
Gene Name: | SUMO-activating enzyme subunit 1 |
Synonyms: | Ubiquitin-like 1-activating enzyme E1A, Sae1, SUMO-activating enzyme subunit 1, Uble1a, Aos1, Sua1, N-terminally processed, Ubl1a1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse SAE1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 39 kDa |
Observed MW. | 39 kDa |
Uniprot ID: | Q9UBE0, Q9R1T2, Q6AXQ0 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis |
IHC/IF | Human, Mouse, Rat | 1: 750-1: 3000 | brain, breast cancer, colon, colon cancer, lung, small intestine, testis |
Background
SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Images
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Western blot analysis of SAE1 (GB111191) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human breast cancer using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded human colon cancer using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded human colon using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse small intestine using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded rat brain using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded rat colon using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunofluorescence of paraffin embedded human breast cancer using SAE1 (GB111191) at dilution of 1: 1100 (400x lens) |
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Immunofluorescence of paraffin embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1100 (400x lens) |
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Immunofluorescence of paraffin embedded mouse lung using SAE1 (GB111191) at dilution of 1: 1100 (400x lens) |
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Immunofluorescence of paraffin embedded rat brain using SAE1 (GB111191) at dilution of 1: 1100 (400x lens) |
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Immunofluorescence of paraffin embedded rat testis using SAE1 (GB111191) at dilution of 1: 1100 (300x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
