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| Reactivity: | M & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Sciellin |
Synonyms: | SCEL, sciellin |
Immunogen: | Recombinant protein corresponding to Mouse SCEL |
Uniprot ID: | Q9EQG3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Membrane |
Predicted MW. / Observed MW. | 73 kDa / 68 kDa |
Product Usage Information
WB | Mouse | 1: 800-1: 1200 | liver, colon, testis |
IHC/IF | Human | 1: 1500-1: 3000 | tonsil, esophageal cancer |
Background
May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope (By similarity).
Images
| Western blot analysis of SCEL (GB114396) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using SCEL (GB114396) at dilution of 1: 3000 |
| Immunofluorescence analysis of paraffin embedded human esophageal cancer using SCEL (GB114396) at dilution of 1: 1500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
