Your shopping cart is empty!
Anti-SIRP alpha Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SIRP alpha rabbit polyclonal antibody |
Gene Name: | Tyrosine-protein phosphatase non-receptor type substrate 1 |
Synonyms: | Sirpa, SIRPA, BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP, SIRPα, Signal-regulatory protein alpha, Signal regulatory protein alpha |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse SIRPα |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. | 56 kDa |
Observed MW. | 100 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | brain, cerebellum, cerebral cortex |
IHC/IF | Mouse, Rat | 1: 500-1: 3600 | kidney, lung, spleen |
Background
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
Images
|
|
Western blot analysis of SIRPA (GB11914) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded mouse kidney using SIRPA (GB11914) at dilution of 1: 3600 (250x lens) |
 | Immunohistochemistry of paraffin embedded rat lung using SIRPA (GB11914) at dilution of 1: 3600 (250x lens) |
|
|
Immunofluorescence of paraffin embedded mouse lung using SIRPA (GB11914) at dilution of 1: 1000 (300x lens) |
|
|
Immunofluorescence of paraffin embedded rat spleen using SIRPA (GB11914) at dilution of 1: 1000 (300x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
