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Anti-SIRT3 Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | NAD-dependent protein deacetylase sirtuin-3 |
Synonyms: | SIR2 like protein 3, hSIRT3, SIR2L3, SIRT3 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse sirt3 |
Uniprot ID: | Q8R104 |
Isotype: | lgG |
Purity: | Affinity purification |
Predicted MW / Observed MW | 44, 28 kDa/ 28 kDa |
Subcellular location: | Mitochondrion, Cytoplasm |
Product Usage Information
WB | Mouse, Rat | 1: 300-1: 400 | liver, lung |
IHC/IF | Mouse, Rat | 1: 100-1: 200 | heart, spleen, skeletal muscle |
Background
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation.
Images
| Western blot analysis of SIRT3 (GB115590) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded mouse heart using SIRT3 (GB115590) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded mouse skeletal muscle using SIRT3 (GB115590) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded mouse skeletal muscle using SIRT3 (GB115590) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded rat heart using SIRT3 (GB115590) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded rat skeletal muscle using SIRT3 (GB115590) at dilution of 1:150 |
| Immunohistochemistry analysis of paraffin embedded rat spleen using SIRT3 (GB115590) at dilution of 1:150 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
