Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | SLBP rabbit polyclonal antibody |
Gene Name: | Histone RNA hairpin-binding protein |
Synonyms: | Histone stem-loop-binding protein, Slbp, Hbp, stem loop binding protein |
Immunogen: | Recombinant protein corresponding to Mouse SLBP |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 32 kDa |
Observed MW. | 43 kDa |
Uniprot ID: | Q14493, P97440 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lymph node, testis |
IHC/IF | Mouse, Rat | 1: 600-1: 1400 | lymph node, ovary, testis |
Background
This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1.
Images
Western blot analysis of SLBP (GB111978) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded mouse testis using SLBP (GB111978) at dilution of 1: 700 (300x lens) | |
Immunohistochemistry of paraffin embedded rat ovary using SLBP (GB111978) at dilution of 1: 700 (400x lens) | |
Immunohistochemistry of paraffin embedded rat testis using SLBP (GB111978) at dilution of 1: 700 (300x lens) | |
Immunofluorescence of paraffin embedded mouse lymph node using SLBP (GB111978) at dilution of 1: 650 (400x lens) | |
Immunofluorescence of paraffin embedded mouse testis using SLBP (GB111978) at dilution of 1: 650 (300x lens) | |
Immunofluorescence of paraffin embedded rat lymph node using SLBP (GB111978) at dilution of 1: 650 (400x lens) | |
Immunofluorescence of paraffin embedded rat testis using SLBP (GB111978) at dilution of 1: 650 (300x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |