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Anti-SMURF 2 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SMURF 2 rabbit polyclonal antibody |
Gene Name: | E3 ubiquitin-protein ligase SMURF2 |
Synonyms: | HECT-type E3 ubiquitin transferase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, Smurf2 |
Immunogen: | Recombinant protein corresponding to Mouse SMURF 2 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 86 kDa |
Observed MW. | 86 kDa |
Uniprot ID: | Q9HAU4, A2A5Z6 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis |
Background
SMURF2 is a E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Images
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Western blot analysis of smurf2 (GB111089) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
