Your shopping cart is empty!
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Synaptotagmin-1 |
Synonyms: | Synaptotagmin I, SytI, p65, DKFZp781D2042, FLJ42519, SVP65, Syt2 |
Immunogen: | Recombinant protein corresponding to Mouse SYT1 |
Uniprot ID: | P46096 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 47 kDa / 60 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, cerebral cortex, cerebellum |
Background
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin's Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported.
Images
| Western blot analysis of SYT1 (GB121130) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
