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Anti-Sarcomeric Alpha Actinin Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Alpha-actinin-2 |
Synonyms: | ACTN2, CMD1AA, CMH23, Actinin alpha 2, alpha Actinin 2,Actn2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse alpha Actinin 2 |
Uniprot ID: | Q9JI91 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 104 kDa / 104 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 1000-1: 2000 | heart, lung, skeletal muscle |
IHC/IF | Mouse, Rat | 1: 500-1: 2000 | heart, Skeletal muscle |
IEM | Mouse, Rat | 1: 50 | heart |
Background
Alpha-actinin 2 is a protein which in humans is encoded by the ACTN2 gene. This gene encodes an alpha-actinin isoform that is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs. The primary function of alpha-actinin 2 is to crosslink filamentous actin molecules and titin molecules from adjoining sarcomeres at Z-discs, a function that is modulated by phospholipids. Alpha-actinin 2 also functions in docking signalling molecules at Z-discs, and additional studies have also implicated alpha-actinin 2 in the binding of cardiac ion channels, Kv1.5 in particular.
Images
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Western blot analysis of Sarcomeric α Actinin (GB11555) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded mouse heart using Sarcomeric α Actinin (GB11555) at dilution of 1: 1000 (200x lens) |
| Immunohistochemistry of paraffin embedded mouse skeletal muscle using Sarcomeric α Actinin (GB11555) at dilution of 1:1000 (200x lens) |
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Immunohistochemistry of paraffin embedded rat heart using Sarcomeric α Actinin (GB11555) at dilution of 1: 1000 (200x lens) |
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Immunohistochemistry of paraffin embedded rat skeletal muscle using Sarcomeric α Actinin (GB11555) at dilution of 1:1000 (200x lens) |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse heart using alpha Actinin 2 (GB11555) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
 | Immunoelectron microscopy analysis of LR white resin-embedded mouse heart using alpha Actinin 2 (GB11555) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
 | Immunoelectron microscopy analysis of LR white resin-embedded mouse heart using alpha Actinin 2 (GB11555) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
