Your shopping cart is empty!
| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Smad4 rabbit polyclonal antibody |
Gene Name: | Mothers against decapentaplegic homolog 4 |
Synonyms: | DPC4, JIP, MADH4, MYHRS, SMAD family member 4 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Smad4 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 60 kDa |
Observed MW. | 70 kDa |
Uniprot ID: | P97471, Q13485 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, stomach |
Background
Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).
Images
|
|
Western blot analysis of Smad4 (GB11174) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
