Reactivity: | H & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | TEAD3 rabbit polyclonal antibody |
Gene Name: | Transcriptional enhancer factor TEF-5 |
Synonyms: | DTEF 1, ETFR 1, TEA domain family member 3, TEAD3, TEAD5, TEF5, ETF-related factor 1, Tcf13r2 |
Immunogen: | Recombinant protein corresponding to Mouse TEAD3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 49 kDa |
Observed MW. | 49 kDa |
Uniprot ID: | Q99594, P70210 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 500-1: 1000 | MCF7, HepG2, Jurkat |
IHC/IF | Human, Mouse, Rat | 1: 600-1: 1400 | heart, lung, lung cancer |
Background
TEAD3, others named ranscriptional enhancer factor 5(TEF5), a member of family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involve in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in placenta and choriocarcinoma cells(JEG-3), but low in Hela, HepG2 and MCF-7. This is a rabbit polyclonal anti-TEAD3 antibody raised against part of C-terminal chain of human TEAD3.
Images
Western blot analysis of Tead3 (GB111974) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded mouse lung using TEAD3 (GB111974) at dilution of 1: 700 (400x lens) | |
Immunohistochemistry of paraffin embedded rat heart using TEAD3 (GB111974) at dilution of 1: 700 (400x lens) | |
Immunohistochemistry of paraffin embedded rat lung using TEAD3 (GB111974) at dilution of 1: 700 (400x lens) | |
Immunofluorescence of paraffin embedded human lung cancer using TEAD3 (GB111974) at dilution of 1: 700 (400x lens) | |
Immunofluorescence of paraffin embedded mouse lung using TEAD3 (GB111974) at dilution of 1: 700 (400x lens) | |
Immunofluorescence of paraffin embedded rat lung using TEAD3 (GB111974) at dilution of 1: 700 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |