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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | TFIIB rabbit polyclonal antibody |
Gene Name: | Transcription initiation factor IIB |
Synonyms: | General transcription factor TFIIB, Gtf2b, RNA polymerase II alpha initiation factor, S300 II, RNA polymerase II transcription factor IIB, General transcription factor IIB |
Immunogen: | Recombinant protein corresponding to Mouse TFIIB |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 35 kDa |
Observed MW. | 35 kDa |
Uniprot ID: | Q00403, P62915, P62916 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HepG2, K562, HeLa, Spleen, kidney, lung |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1500 | breast cancer, lung, testis |
Background
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA .Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex .Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle . Associates with chromatin to core promoter- specific regions .
Images
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Western blot analysis of tfiib (GB11995) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded mouse lung using tfiib (GB11995) at dilution of 1: 900 (250x lens) |
 | Immunohistochemistry of paraffin embedded rat lung using tfiib (GB11995) at dilution of 1: 900 (250x lens) |
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Immunohistochemistry of paraffin embedded rat testis using tfiib (GB11995) at dilution of 1: 900 (250x lens) |
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Immunofluorescence of paraffin embedded human breast cancer using tfiib (GB11995) at dilution of 1: 500 (300x lens) |
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Immunofluorescence of paraffin embedded rat lung using tfiib (GB11995) at dilution of 1: 500 (300x lens) |
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Immunofluorescence of paraffin embedded rat testis using tfiib (GB11995) at dilution of 1: 500 (250x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
