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Recombinant Anti-TLR4 Mouse mAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Toll-like receptor 4 |
Synonyms: | Lps,TLR4, ARMD10, CD284, TLR-4, TOLL, toll like receptor 4 |
Immunogen: | Recombinant protein corresponding to Mouse TLR4 |
Uniprot ID: | Q9QUK6 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 95 kDa / 95 kDa |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | esophageal carcinoma, tonsil, Inflammatory model induced by lps(6h) spleen |
Background
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS).Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate.In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42.In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36.
Images
| IHC analysis of TLR4(GB15186). Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of TLR4(GB15186). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of TLR4(GB15186). Sample: Mouse Inflammatory model induced by lps(6h) spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of TLR4(GB15186). Sample: Rat model of antigen stimulation spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
