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Anti-TMS1/ASC Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Apoptosis-associated speck-like protein containing a CARD |
Synonyms: | ASC, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human TMS1/ASC |
Uniprot ID: | Q9ULZ3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus |
Predicted MW. / Observed MW. | 22 kDa / 22 kDa |
Product Usage Information
WB | Human | 1: 300-1: 600 | THP-1, MCF7 |
IHC/IF | Human | 1 :400-1: 800 | tonsil, liver, colon |
Background
Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response, acts as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of pro-inflammatory cytokines .
Images
| Western blot analysis of ASC (GB115270) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using ASC (GB115270) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using ASC (GB115270) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded human liver using ASC (GB115270) at dilution of 1: 1800 |
| Immunohistochemistry analysis of paraffin embedded human colon using ASC (GB115270) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded human liver using ASC (GB115270) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded human colon using ASC (GB115270) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
