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Anti-TOMM20 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Mitochondrial import receptor subunit TOM20 |
Synonyms: | Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tomm20, Translocase of outer mitochondrial membrane 20 homolog type II, KIAA0016, MAS20, Tom20, MGC117367 |
Immunogen: | Recombinant protein corresponding to Mouse TOMM20 |
Uniprot ID: | Q9DCC8 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. / Observed MW. | 16 kDa / 16 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | liver, brain, cerebral cortex |
ICC/IF | Human | 1: 1000-1: 2000 | HeLa, HepG2 |
Background
TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.
Images
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WB analysis of .Tom20 (GB111481). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of.Tom20 (GB111481)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.. |
| ICC analysis of.Tom20 (GB111481)red. Sample: HepG2, 4% PFA (G1101) 20min. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
