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Anti-TOP1MT Rabbit pAb (100 μl)
| Reactivity: | R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | DNA topoisomerase I, mitochondrial |
Synonyms: | Topoisomerase I mitochondrial, 2900052H09Rik |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse TOP1MT |
Uniprot ID: | Q8R4U6 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 69 kDa / 66 kDa |
Product Usage Information
WB | Rat | 1: 2000-1: 3000 | colon, lung, pancreas, ovary |
Background
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Images
| Western blot analysis of TOP1MT (GB115253) at dilution of 1: 2000 |
| Western blot analysis of TOP1MT (GB115253) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
