Reactivity: | R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | TTLL10 rabbit polyclonal |
Gene Name: | Protein polyglycylase TTLL10 |
Synonyms: | Tubulin--tyrosine ligase-like protein 10, Ttll10, TTLL5, Inactive polyglycylase TTLL10 |
Immunogen: | Recombinant protein corresponding to Mouse TTLL10 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 79 kDa |
Observed MW. | 72 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Rat | 1: 500-1: 1000 | testis, stomach |
Background
Polyglycylase which modifies both tubulin and non-tubulin proteins, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues of target proteins. Polyglycylates alpha-tubulin and beta-tubulin, but is not able to initiate glycylation and only has activity toward monoglycylated tubulin. Has the ability to polyglycylate non-tubulin proteins such as NAP1; in this case it can initiate glycylation and does not require preliminary monoglycylation by another glycylase.
Images
Western blot analysis of TTLL10 (GB112961) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |