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Anti-Thioredoxin / TRX Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Thioredoxin rabbit polyclonal antibody |
Gene Name: | Thioredoxin |
Synonyms: | Surface associated sulphydryl protein, TRDX, Thioredoxin1, DKFZp686B1993, MGC61975, Trx, ATL-derived factor, ADF, Txn, Txn1, SASP, ATL-derived factor, zgc:92903 |
Immunogen: | Recombinant protein corresponding to Mouse Thioredoxin |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 12 kDa |
Observed MW. | 12 kDa |
Uniprot ID: | P10599, P10639, P11232 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500- 1: 1000 | colon, lung, liver |
Background
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cystein residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53 , NF-kB and AP-1 (T1-151).
Images
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Western blot analysis of Thioredoxin (GB11993) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
