Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Thymidylate synthase |
Synonyms: | HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Thymidylate Synthase |
Uniprot ID: | P07607 |
Isotype: | lgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 35 kDa / 36 kDa |
Product Usage Information
WB | Human | 1: 300-1: 400 | HeLa, Jurkat |
Background
Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Images
WB analysis of Thymidylate Synthase (GB115510). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |