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Anti-Tropomodulin 3 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Tropomodulin-3 |
Synonyms: | TMOD3, tropomodulin 3 (ubiquitous), Ubiquitous tropomodulin, UTMOD, U-Tmod |
Immunogen: | Recombinant protein corresponding to Mouse Tropomodulin 3 |
Uniprot ID: | Q9JHJ0 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 40 kDa / 40 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, kidney |
ICC/IF | Human, Mouse | 1: 100-1: 400 | HeLa, NIH3T3 |
Background
Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton.
Images
| WB analysis of Tropomodulin 3(GB113249). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of Tropomodulin 3(GB113249)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of Tropomodulin 3(GB113249)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
