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Anti-UBE2I / UBC9 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | SUMO-conjugating enzyme UBC9 |
Synonyms: | RING-type E3 SUMO transferase UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, mUBC9, Ubiquitin carrier protein I, Ubiquitin-protein ligase I, Ube2i, Ubc9, Ubce2i, Ubce9, UBE2I / UBC9 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse UBE2I / UBC9 |
Uniprot ID: | P63280 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 18 kDa / 18 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | spleen, thymus, lung, brain |
IHC/IF | Human | 1: 1000-1: 2000 | B-cell lymphoma |
Background
UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues.
Images
| Western blot analysis of UBC9 (GB111874) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human B-cell lymphoma using UBC9 (GB111874) at dilution of 1: 2300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
