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Anti-UBP43 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Ubl carboxyl-terminal hydrolase 18 |
Synonyms: | 43 kDa ISG15-specific protease, ISG15-specific-processing protease, Ubl thioesterase 18, Usp18, UBP43 |
Immunogen: | Recombinant protein corresponding to Mouse UBP43 |
Uniprot ID: | Q9WTV6 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 42 kDa / 42 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, HepG2, A-431 |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, heart |
Background
USP18(UBP43) is a 43 kDa protein that is known as an ISG15-deconjugating enzyme and an inhibitor of type I IFN signaling independent of its enzyme activity. USP18 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. It can be detected two bands(apparent MW of 38 and 35 kDa), the faster migrating one results from proteolytic processing in human primary hepatocytes.
Images
| Western blot analysis of ubp43 (GB111923) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse brain using ubp43 (GB111923) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraffin embedded mouse heart using ubp43 (GB111923) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraffin embedded mouse brain using ubp43 (GB111923) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
