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| Reactivity: | H,M,R & H |
| Applications: | WB & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Transitional endoplasmic reticulum ATPase |
Synonyms: | TER ATPase, 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, VCP |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse VCP |
Uniprot ID: | Q01853 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 89 kDa / 97 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, brain |
ICC/IF | Human | 1: 50-1: 100 | HeLa |
Background
Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2).
Images
| WB analysis of VCP(GB121155). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| ICC analysis of VCP(GB121155)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
