Reactivity: | H,M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | VPS25 rabbit polyclonal antibody |
Gene Name: | Vacuolar protein-sorting-associated protein 25 |
Synonyms: | DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25 |
Immunogen: | Recombinant protein corresponding to Mouse VPS25 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 21 kDa |
Observed MW. | 21 kDa |
Uniprot ID: | Q9CQ80 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | heart, kidney, liver |
Background
VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).
Images
Western blot analysis of VPS25 (GB114031) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |