Anti-Vinculin Mouse mAb (100 μl)

Anti-Vinculin Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB121328-100
Availability: In Stock
$259.00
-+
Reactivity: H,M,R    
Applications: WB    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name:

Vinculin

Synonyms:

Metavinculin, Vcl, CMD1W, CMH15, MVCL, MV, VINC

Immunogen:

Recombinant protein corresponding to Human Vinculin

Uniprot ID:

P18206

Isotype:

IgG2a,k

Purity:

Affinity purification

Predicted MW. / Observed MW.

117 kDa / 124 kDa




Product Usage Information

WB

Human, Mouse ,Rat

1: 500-1: 1000

lung , NIH/3T3 , uterine




Background

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion.




Images

01 GB121328_Vinculin_3E9H5_d1000_WB_WM.png

WB analysis of Vinculin(GB121328).

Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.




Storage

StorageStore at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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