Your shopping cart is empty!
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | WEE2 rabbit polyclonal |
Gene Name: | Wee1-like protein kinase 2 |
Synonyms: | WEE1 homolog 2 (S. pombe), Wee1 like protein kinase 1B, WEE1B, Wee1B kinase, WEE2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse WEE2 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 62 kDa |
Observed MW. | 62 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 300-1: 600 | Jurkat, A-431, U-20S, HUVEC |
Background
WEE2, also named as WEE1B, belongs to the protein kinase superfamily. Ser/Thr protein kinase family and WEE1 subfamily. It phosphorylates and inhibits CDK1. WEE2 may act as a negative regulator of entry into mitosis (G2 to M transition). WEE2 is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Taking the long double-stranded RNA-mediated RNA interference approach, down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. Overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.
Images
 | Western blot analysis of WEE2 (GB113669) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
