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| Reactivity: | H,R & H |
| Applications: | WB & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | XLF rabbit polyclonal |
Gene Name: | Non-homologous end-joining factor 1 |
Synonyms: | Protein cernunnos, XRCC4-like factor, NHEJ1, XLF |
Immunogen: | Recombinant protein corresponding to Human XLF |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 33 kDa |
Observed MW. | 43 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Rat | 1: 500-1: 1000 | liver |
IF | Human | 1: 300-1: 600 | colon cancer |
Background
XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.
Images
 | Western blot analysis of XLF (GB113018) at dilution of 1: 1000 Lane 1: PC-3 cell lysate Lane 2: HeLa cell lysate Lane 3: K562 cell lysate Lane 4: Rat liver tissue lysate |
| Immunofluorescent analysis of paraformaldehyde-fixed human colon cancer using GB113018) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
