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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Y14 rabbit polyclonal antibody |
Gene Name: | RNA-binding protein 8A |
Synonyms: | RNA-binding motif protein 8A, Ribonucleoprotein RBM8A, Rbm8a, Rbm8, Binder of OVCA1 1, HSPC 114, BOV 1B, ZNRP, RNA-binding protein Y14 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Y14 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 20 kDa |
Observed MW. | 20 kDa |
Uniprot ID: | Q9Y5S9, Q9CWZ3, Q27W01 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, kidney |
IHC/IF | Human, Mouse, Rat | 1: 300-1: 900 | adrenal gland, brain, hippocampus, kidney, liver cancer, testis |
Background
This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events.
Images
| Western blot analysis of RBM8A (GB111729) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded mouse adrenal gland using RBM8A (GB111729) at dilution of 1: 300 (300x lens) |
| Immunohistochemistry of paraffin embedded mouse brain using RBM8A (GB111729) at dilution of 1: 300 (300x lens) |
| Immunohistochemistry of paraffin embedded mouse testis using RBM8A (GB111729) at dilution of 1: 300 (300x lens) |
| Immunohistochemistry of paraffin embedded rat adrenal gland using RBM8A (GB111729) at dilution of 1: 300 (300x lens) |
| Immunohistochemistry of paraffin embedded rat hippocampus using RBM8A (GB111729) at dilution of 1: 300 (300x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using RBM8A (GB111729) at dilution of 1: 300 (300x lens) |
| Immunohistochemistry of paraffin embedded rat testis using RBM8A (GB111729) at dilution of 1: 300 (300x lens) |
| Immunofluorescence of paraffin embedded human liver cancer using RBM8A (GB111729) at dilution of 1: 300 (400x lens) |
| Immunofluorescence of paraffin embedded mouse testis using RBM8A (GB111729) at dilution of 1: 300 (300x lens) |
 | Immunofluorescence of paraffin embedded rat hippocampus using RBM8A (GB111729) at dilution of 1: 300 (200x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
