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Anti-ZO1 tight junction protein Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Tight junction protein ZO-1 |
Synonyms: | TJP1, ZO-1, Tight junction protein 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ZO-1 |
Uniprot ID: | P39447 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cell junction |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1200 | colon, duodenum, small intestine |
Background
Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton.The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation.Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells.Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling.With Tjp2 and TJjp3, participates in the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus.
Images
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Immunohistochemistry analysis of paraffin embedded human colon using ZO-1 (GB11195) at dilution of 1: 500 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse duodenum using ZO1 (GB11195) at dilution of 1: 800 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat small intestine using ZO1 (GB11195) at dilution of 1: 800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
