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Anti-ZO1 tight junction protein Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ZO1 tight junction protein rabbit polyclonal antibody |
Gene Name: | Tight junction protein ZO-1 |
Synonyms: | TJP1, ZO-1, Tight junction protein 1 |
Immunogen: | Recombinant protein corresponding to Mouse ZO-1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cell junction |
Uniprot ID: | Q07157, P39447 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Human, Mouse, Rat | 1: 200-1: 1200 | brain, colon cancer, duodenum, jejunum, small intestine |
Background
Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton.The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation.Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells.Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling.With Tjp2 and TJjp3, participates in the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus.
Images
| Immunohistochemistry of paraffin embedded human brain using ZO-1 (GB111981) at dilution of 1:1200 (100x lens) |
| Immunohistochemistry of paraffin embedded mouse small intestine using ZO-1 (GB111981) at dilution of 1:1200 (200x lens) |
| Immunohistochemistry of paraffin embedded rat brain using ZO-1 (GB111981) at dilution of 1:1200 (100x lens) |
| Immunohistochemistry of paraffin embedded rat jejunum using ZO-1 (GB111981) at dilution of 1:1200 (100x lens) |
| Immunohistochemistry of paraffin embedded rat small intestine using ZO-1 (GB111981) at dilution of 1:1200 (200x lens) |
| Immunofluorescence of paraffin embedded human colon cancer using ZO-1 (GB111981) at dilution of 1:800 (40x lens) |
| Immunofluorescence of paraffin embedded mouse duodenum using ZO-1 (GB111981) at dilution of 1:800 (100x lens) |
| Immunofluorescence of paraffin embedded mouse small intestine using ZO-1 (GB111981) at dilution of 1:800 (100x lens) |
| Immunofluorescence of paraffin embedded rat small intestine using ZO-1 (GB111981) at dilution of 1:800 (100x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
