Reactivity: | H,M,R & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Mouse |
Clonality: | Monoclonal |
Gene Name: | Tubulin alpha-1A chain |
Synonyms: | TUBA1A, B-ALPHA-1, LIS3, TUBA3, tubulin alpha 1a,TUBA1B, K-ALPHA-1, tubulin alpha 1b |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse alpha Tubulin |
Uniprot ID: | P68369 |
Isotype: | IgG2b,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoskeleton |
Predicted MW. / Observed MW. | 55 kDa / 55 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 2000-1: 4000 | brain, cerebral cortex, hippocampus |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, fibrous tumor, kidney |
Background
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.
Images
Western blot analysis of a-Tubulin (GB12200) at dilution of 1: 2000 | |
Immunofluorescence of paraffin embedded human fibrous tumor using α-tubulin (GB12200) at dilution of 1: 2000 (200x lens) | |
Immunofluorescence of paraffin embedded human kidney using α-tubulin (GB12200) at dilution of 1: 2000 (200x lens) | |
Immunofluorescence of paraffin embedded mouse brain using α-tubulin (GB12200) at dilution of 1: 2000 (200x lens) | |
Immunofluorescence of paraffin embedded mouse kidney using α-tubulin (GB12200) at dilution of 1: 2000 (200x lens) | |
Immunofluorescence of paraffin embedded mouse lung using α-tubulin (GB12200) at dilution of 1: 2000 (200x lens) | |
Immunofluorescence of paraffin embedded rat kidney using α-tubulin (GB12200) at dilution of 1: 2000 (200x lens) | |
Immunofluorescence of paraffin embedded rat lung using α-tubulin (GB12200) at dilution of 1: 2000 (200x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |